On the other hand frozen sections provided more tissue architectural details but frequently hampered by freezing artifacts. Imprint cytology definition of imprint cytology by. Intraoperative imprint cytology and frozen section. Two intraoperative margin assessment methods are frequently reported in the literature. Avoid pressing tissue to the cryostat stage which can result in adhesion of the tissue to the stage, especially in fatty.
Hasenburg a, ledet sc, ardaman t, levy t, kieback dg. A college of american pathologists qprobes study of frozen sections from various body sites found the thyroid and paraaccepted for publication august 8, 2005. The aim of our study was to perform a direct comparison between ic and fs on the same slns of bc cases operated in a single center by the same surgical team. Here we directly compared the sensitivity of these methods prospectively. The study of cellular morphology or of tissue diseases after a clinical specimen, e. Clinical research paper clinical significance of mskcc. Accuracy of intraoperative imprint cytology in breast.
Several problem areas exist in intraoperative evaluation of sentinel nodes whether it be by frozen sectioning or by imprint cytology. Between october 1997 and june 2004, thoracic lesions in 622 patients underwent biopsy using 19gauge coaxial guiding needles and 20gauge biopsy needles under computed tomography guidance. Impact of immediate evaluation of touch imprint cytology. Imprint cytology article about imprint cytology by the. Sentinel lymph node sln biopsy is the gold standard for surgical staging of the axilla in breast cancer bc. Quality was assessed by tive imprint cytology, frozen section analysis e. Evaluation of fine needleaspiration and imprint cytology in the early diagnosis of breastlesions with histopathological correlation. Fineneedle aspiration versus frozen section in the. Imprint cytology versus frozen section analysis for. Breast employing imprint preparation along with the actual frozen section facilitate the appreciation of cytologic detail. Fsa is the intraoperative technique that has been most widely used to analyze breast tumor excisions.
Insular carcinoma is another rare tumor, arising from. Various methods have been used for intraoperative analysis of slns, including frozen section fs, touch imprint cytology tic, scrapescrimp cytology sc and. Evaluation of lymph nodes in squamous cell carcinoma of. Sentinel lymph node metastasis of breast cancer is evaluated by frozen section fs or touch imprint cytology tic. A comparison of touch imprint cytology and mohs frozensection histology in the evaluation of mohs micrographic surgical margins. Comparing touch imprint cytology, frozen section analysis, and cytokeratin immunostaining for intraoperative evaluation of axillary sentinel lymph nodes in breast cancer. Thus, imprint cytology plays a significant role in the quick diagnosis of the lesion. Intraoperative examination of the sentinel lymph node for. Safai a, razeghi a, monabati a, azarpira n, talei a. Intraoperative sentinel lymph node examination by frozen. Use of touch imprint cytology as a simple method to enrich. Figure 4c illustrates that by being extremely delicate with the brush. Towards this end, a prospective study of 102 patients undergoing mastectomy or sector resection with sn biopsy was conducted. Thyroid malignancy can be found on 5% of thyroid nodules.
The evaluation of frozen section combined with imprint cytology is very useful, because this examination significantly showed high accuracy in diagnosing thyroid malignancy. Frozen section overview and application linkedin slideshare. Imprint, frozen section and permanent, embedded section positive figures 1 and 2 32 24 imprint negative, frozen section and permanent, embedded section positive 16 14. Conventional procedures for the intraoperative assessment of breast cancer sentinel lymph nodes slns are frozen section fs and touch imprint cytology tic. Mutation analysis the relative percentage of the mutant allele in a given.
Touch impression cytology versus frozen section as intraoperative consultation diagnosis ahmareen khalid and anwar ul haque department of pathology, pakistan institute of medical sciences, islamabad. Frozen section analysis versus imprint cytology for this. Comparison of frozen section and touch imprint cytology. Role of intraoperative pathology consultation in skeletal. Three hundred fortytwo slns were removed from 79 patients. We conclude that both touch imprint cytology and frozen sections are dependable intraoperative consultation diagnostic modalities. Imprint cytology ic has also been suggested as a less expensive and equally accurate alternative to fs. Imprint smears prepared from fresh surgical specimens give excellent cytological clarity. Frozensection analysis fs, because of its high specificity, was useful in reducing the need for completion thyroidectomy in patients in whom fineneedle aspiration cytology fnac findings were negative or nondiagnostic and in whom other clinical factors suggested the. Intraoperative evaluation consisted of touch imprint cytology tic, frozen section fs analysis, scrape cytology or combination of these methods with own advantages and disadvantages. The aim of this study was to evaluate the optimal approach by comparing osna assay, fs, and tic. Scrape cytology is a modification of imprint cytology that provides rapid and cheap intraoperative identification of parathyroid tissue, but its reliability remains controversial.
Comparing touch imprint cytology, frozen section analysis. General principles of cytology is identify inflammation vs. Intraoperative identification of parathyroid tissue is crucial during parathyroid surgery. Frozen section fs remains the most popular means of intraoperative sln diagnosis. Imprint cytology definition of imprint cytology by the. Frozen section examination of the thyroid and parathyroids is not as straightforward as it may seem. Accuracy of frozensection combined with imprint and fine. Cost effectiveness, rapid results and simplicity are the further criteria promoting it to be a better option when compared with other techniques such as frozen sections. Imprint cytology improves accuracy of computed tomography. Woo, qunzi zhao, ping wang, pintong huang, lirong chen, xin li, kanlun xu, ying yong, stephanie sungeun yang, and jianyu rao 2017 fineneedle aspiration versus frozen section in the evaluation of malignant thyroid nodules in patients with the diagnosis of suspicious for malignancy or malignancy by fineneedle aspiration. Touch imprint and crash preparation intra operative cytology versus frozen section in thyroid nodule ali chehrei, 1 mojtaba ahmadinejad, 2 sayyed abbas tabatabaee, 3 sayyed mozaffar hashemi, 3 mahsa kianinia, 4 shahin fateh, 5 and mohammad hossein sanei 6. Frozen section is the most common tool, but is timeconsuming and expensive.
The diagnostic accuracy of imprint cytology in breast. The efficacy of touch imprint cytology in the diagnosis of various pathological processes was evaluated and compared with frozen section in intraoperative consultation. This study optimized the use of tic and fs in the intraoperative detection of breast slns based on the memorial sloan kettering cancer center mskcc nomogram. Intraoperative frozensection analysis for thyroid nodules. Fine needle aspiration biopsy fnab was done preoperatively while frozen section fs and imprint cytology ic should be. Touch imprint cytology provided better cellular morphology and fewer artifacts. A frozen section is difficult to execute and interpret in breast specimen due to high fat content and atypical proliferation at the margins due to cautery artifact. However, which of the two methods is superior remains controversial. Evaluation of lymph nodes in squamous cell carcinoma of the cervix.
Touch impression cytology versus frozen section as. Metastatic involvement of pelvic lymph nodes lns is the most important negative. When a frozen section is performed, the histopathology laboratory will initially be notified that the surgical specimen is on its way from theatre, allowing time to prepare to receive the sample. Touch imprint and crash preparation intra operative. Cont george papanicolaou claimed to be the father of clinicalgeorge papanicolaou claimed to be the father of clinical cytology. Imprint cytology versus frozen section analysis for intraoperative assessment of sentinel lymph node in breast cancer thalia petropoulou, 1 antonia kapoula, 2 aikaterini mastoraki, 3 aikaterini politi, 2 eleni spanidoukarvouni, 2 ioannis psychogios, 1 ioannis vassiliou, 1 and nikolaos arkadopoulos 3. The efficacy of touch imprint cytology in the diagnosis of various pathological processes was evaluated and compared with frozen section in. The first area is the poor sensitivity of detecting. Frozen section analysis versus imprint cytology for assessment of safety margins in breast conservation surgery 0 2 4 6 8 10 12 14 clinical tumor size 2 methods. Compared with h and e stained paraffin sections, ic and fs had an overall accuracy of 93.
Well differentiated carcinomas often cannot be diagnosed on frozen section, regardless of experience of pathologist intraoperative cytology may be useful to detect nuclear features of papillary carcinoma mod pathol 2000. Imprint cytology versus frozen section analysis for intraoperative assessment of sentinel lymph node in breast cancer thalia petropoulou,1 antonia kapoula,2 aikaterini mastoraki,3 aikaterini politi,2 eleni spanidoukarvouni,2 ioannis psychogios,1 ioannis vassiliou,1 nikolaos arkadopoulos3 12nd department of surgery, 2department of pathology, aretaieion university hospital, 34th department of. The aim of the present study was to investigate whether imprint cytology can improve the diagnostic accuracy of computed tomographyguided transthoracic core biopsy. It is used in some settings as an adjunct or alternative to fine needle aspiration biopsy, frozen sectioning, and. Cytology is a joint effort between the clinician andcytology is a joint effort between the clinician and cytologist. The discipline includes cytogenics, cytochemistry, and microscopic anatomy, which involve investigations employing various microscopes, such as light, phase, interference, and electron microscopes. Accuracy of intraoperative frozen section analysis of breast cancer lumpectomy bed margins. Achieving negative surgical margins is critical to minimizing the risk of tumor recurrence in patients undergoing breast conservation surgery bcs for a breast malignancy. Frozen section analysis fs, because of its high specificity, was useful in reducing the need for completion thyroidectomy in patients in whom fineneedle aspiration cytology fnac findings were negative or nondiagnostic and in whom other clinical factors suggested the possibility of malignant disease. The tissue will be examined on arrival by a pathologist, who describes and measures it before selecting the area of most interest to be processed put. Axillary lymph node status is essential in staging breast cancer but unfortunately has relatively high morbidity. Prospective study comparing scrape cytology with frozen. Frozen section of thyroid and parathyroid specimens.
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