Compared with h and e stained paraffin sections, ic and fs had an overall accuracy of 93. Imprint cytology ic has also been suggested as a less expensive and equally accurate alternative to fs. When a frozen section is performed, the histopathology laboratory will initially be notified that the surgical specimen is on its way from theatre, allowing time to prepare to receive the sample. Cont george papanicolaou claimed to be the father of clinicalgeorge papanicolaou claimed to be the father of clinical cytology.
Intraoperative sentinel lymph node examination by frozen. Towards this end, a prospective study of 102 patients undergoing mastectomy or sector resection with sn biopsy was conducted. Accuracy of intraoperative frozen section analysis of breast cancer lumpectomy bed margins. Imprint cytology improves accuracy of computed tomography.
Breast employing imprint preparation along with the actual frozen section facilitate the appreciation of cytologic detail. Evaluation of fine needleaspiration and imprint cytology in the early diagnosis of breastlesions with histopathological correlation. This study optimized the use of tic and fs in the intraoperative detection of breast slns based on the memorial sloan kettering cancer center mskcc nomogram. Fine needle aspiration biopsy fnab was done preoperatively while frozen section fs and imprint cytology ic should be. Role of intraoperative pathology consultation in skeletal. Frozensection analysis fs, because of its high specificity, was useful in reducing the need for completion thyroidectomy in patients in whom fineneedle aspiration cytology fnac findings were negative or nondiagnostic and in whom other clinical factors suggested the. A frozen section is difficult to execute and interpret in breast specimen due to high fat content and atypical proliferation at the margins due to cautery artifact. Metastatic involvement of pelvic lymph nodes lns is the most important negative. Mutation analysis the relative percentage of the mutant allele in a given.
Woo, qunzi zhao, ping wang, pintong huang, lirong chen, xin li, kanlun xu, ying yong, stephanie sungeun yang, and jianyu rao 2017 fineneedle aspiration versus frozen section in the evaluation of malignant thyroid nodules in patients with the diagnosis of suspicious for malignancy or malignancy by fineneedle aspiration. General principles of cytology is identify inflammation vs. Intraoperative identification of parathyroid tissue is crucial during parathyroid surgery. Evaluation of lymph nodes in squamous cell carcinoma of the cervix. Cost effectiveness, rapid results and simplicity are the further criteria promoting it to be a better option when compared with other techniques such as frozen sections.
Quality was assessed by tive imprint cytology, frozen section analysis e. Various methods have been used for intraoperative analysis of slns, including frozen section fs, touch imprint cytology tic, scrapescrimp cytology sc and. Thyroid malignancy can be found on 5% of thyroid nodules. Frozen section is the most common tool, but is timeconsuming and expensive. However, which of the two methods is superior remains controversial. Scrape cytology is a modification of imprint cytology that provides rapid and cheap intraoperative identification of parathyroid tissue, but its reliability remains controversial.
Hasenburg a, ledet sc, ardaman t, levy t, kieback dg. Imprint cytology definition of imprint cytology by. Comparison of frozen section and touch imprint cytology. Intraoperative evaluation of sentinel lymph nodes for.
Clinical research paper clinical significance of mskcc. A college of american pathologists qprobes study of frozen sections from various body sites found the thyroid and paraaccepted for publication august 8, 2005. Imprint, frozen section and permanent, embedded section positive figures 1 and 2 32 24 imprint negative, frozen section and permanent, embedded section positive 16 14. Imprint cytology definition of imprint cytology by the.
Frozen section analysis versus imprint cytology for this. In order to better managed of thyroid nodules, skills to differentiate benign from malignant cases were needed. Frozen section analysis versus imprint cytology for assessment of safety margins in breast conservation surgery 0 2 4 6 8 10 12 14 clinical tumor size 2 methods. Touch imprint and crash preparation intra operative cytology versus frozen section in thyroid nodule ali chehrei, 1 mojtaba ahmadinejad, 2 sayyed abbas tabatabaee, 3 sayyed mozaffar hashemi, 3 mahsa kianinia, 4 shahin fateh, 5 and mohammad hossein sanei 6. Between october 1997 and june 2004, thoracic lesions in 622 patients underwent biopsy using 19gauge coaxial guiding needles and 20gauge biopsy needles under computed tomography guidance. Imprint cytology versus frozen section analysis for. The aim of the present study was to investigate whether imprint cytology can improve the diagnostic accuracy of computed tomographyguided transthoracic core biopsy. Sentinel lymph node metastasis of breast cancer is evaluated by frozen section fs or touch imprint cytology tic. Sentinel lymph node sln biopsy is the gold standard for surgical staging of the axilla in breast cancer bc. Well differentiated carcinomas often cannot be diagnosed on frozen section, regardless of experience of pathologist intraoperative cytology may be useful to detect nuclear features of papillary carcinoma mod pathol 2000. Imprint cytology of sentinel lymph nodes in breast cancer. Safai a, razeghi a, monabati a, azarpira n, talei a. Intraoperative imprint cytology and frozen section.
Frozen section fs remains the most popular means of intraoperative sln diagnosis. The discipline includes cytogenics, cytochemistry, and microscopic anatomy, which involve investigations employing various microscopes, such as light, phase, interference, and electron microscopes. Cytology is a joint effort between the clinician andcytology is a joint effort between the clinician and cytologist. Use of touch imprint cytology as a simple method to enrich. The great advantage of imprint cytology whereas is the preservation of lymphnode material by the technique as up to 50% of the lymphnode can be lost by a routine frozen section. Figure 4c illustrates that by being extremely delicate with the brush. Avoid pressing tissue to the cryostat stage which can result in adhesion of the tissue to the stage, especially in fatty. Conventional procedures for the intraoperative assessment of breast cancer sentinel lymph nodes slns are frozen section fs and touch imprint cytology tic. Imprint cytology versus frozen section analysis for intraoperative assessment of sentinel lymph node in breast cancer thalia petropoulou,1 antonia kapoula,2 aikaterini mastoraki,3 aikaterini politi,2 eleni spanidoukarvouni,2 ioannis psychogios,1 ioannis vassiliou,1 nikolaos arkadopoulos3 12nd department of surgery, 2department of pathology, aretaieion university hospital, 34th department of. Touch impression cytology versus frozen section as. The tissue will be examined on arrival by a pathologist, who describes and measures it before selecting the area of most interest to be processed put. Frozen section of thyroid and parathyroid specimens.
Here we directly compared the sensitivity of these methods prospectively. On the other hand frozen sections provided more tissue architectural details but frequently hampered by freezing artifacts. Frozen section analysis fs, because of its high specificity, was useful in reducing the need for completion thyroidectomy in patients in whom fineneedle aspiration cytology fnac findings were negative or nondiagnostic and in whom other clinical factors suggested the possibility of malignant disease. Fsa is the intraoperative technique that has been most widely used to analyze breast tumor excisions.
Two intraoperative margin assessment methods are frequently reported in the literature. Several problem areas exist in intraoperative evaluation of sentinel nodes whether it be by frozen sectioning or by imprint cytology. Three hundred fortytwo slns were removed from 79 patients. The aim of this study was to evaluate the optimal approach by comparing osna assay, fs, and tic. Touch imprint and crash preparation intra operative. The study of cellular morphology or of tissue diseases after a clinical specimen, e.
Intraoperative examination of the sentinel lymph node for. Insular carcinoma is another rare tumor, arising from. A comparison of touch imprint cytology and mohs frozensection histology in the evaluation of mohs micrographic surgical margins. Intraoperative evaluation consisted of touch imprint cytology tic, frozen section fs analysis, scrape cytology or combination of these methods with own advantages and disadvantages. Frozen section overview and application linkedin slideshare. Evaluation of lymph nodes in squamous cell carcinoma of. The aim of our study was to perform a direct comparison between ic and fs on the same slns of bc cases operated in a single center by the same surgical team. Imprint smears prepared from fresh surgical specimens give excellent cytological clarity. Impact of immediate evaluation of touch imprint cytology. The diagnostic accuracy of imprint cytology in breast.
Intraoperative frozensection analysis for thyroid nodules. The evaluation of frozen section combined with imprint cytology is very useful, because this examination significantly showed high accuracy in diagnosing thyroid malignancy. Prospective study comparing scrape cytology with frozen. Comparing touch imprint cytology, frozen section analysis, and cytokeratin immunostaining for intraoperative evaluation of axillary sentinel lymph nodes in breast cancer. Imprint cytology article about imprint cytology by the. The efficacy of touch imprint cytology in the diagnosis of various pathological processes was evaluated and compared with frozen section in. The efficacy of touch imprint cytology in the diagnosis of various pathological processes was evaluated and compared with frozen section in intraoperative consultation. The first area is the poor sensitivity of detecting. Thus, imprint cytology plays a significant role in the quick diagnosis of the lesion. Imprint cytology versus frozen section analysis for intraoperative assessment of sentinel lymph node in breast cancer thalia petropoulou, 1 antonia kapoula, 2 aikaterini mastoraki, 3 aikaterini politi, 2 eleni spanidoukarvouni, 2 ioannis psychogios, 1 ioannis vassiliou, 1 and nikolaos arkadopoulos 3. Touch imprint cytology provided better cellular morphology and fewer artifacts. Accuracy of frozensection combined with imprint and fine.
Achieving negative surgical margins is critical to minimizing the risk of tumor recurrence in patients undergoing breast conservation surgery bcs for a breast malignancy. Touch impression cytology versus frozen section as intraoperative consultation diagnosis ahmareen khalid and anwar ul haque department of pathology, pakistan institute of medical sciences, islamabad. Imprint cytology and frozen section together provide a quick, safe, and reliable intraoperative provisional tissue diagnosis in skeletal tumors and tumorlike conditions. We conclude that both touch imprint cytology and frozen sections are dependable intraoperative consultation diagnostic modalities. It is used in some settings as an adjunct or alternative to fine needle aspiration biopsy, frozen sectioning, and. Accuracy of intraoperative imprint cytology in breast. Fineneedle aspiration versus frozen section in the. Frozen section examination of the thyroid and parathyroids is not as straightforward as it may seem.
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